Mouse lung tissue lysate, Non-denatured; Abnova

Name: Mouse lung tissue lysate, Non-denatured; Abnova
Brand: Abnova
SKU: 16009585
Price: 109.00 EUR

Marca: Abnova L029W2.200ug

Dettagli aggiuntivi : Peso : 0.02000kg

Codice prodotto. 16009585

Spedizione stimata: 14-06-2024
per visualizzare lo stock disponibile

Effettua il login per acquistare questo articolo Hai bisogno di un conto web ? Registrati oggi stesso!

Descrizione

Western Blotting, Immunoprecipitation

Specifica

Da utilizzare con (applicazione)	Immunoprecipitation, Western Blot
Descrizione	Whole tissue lysate (non-denatured)
Metodo di preparazione	Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Quantità	200 μg
Specie ospite	Mouse

Tissue	Lung
Tampone di lisi	Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. ;Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Analisi di controllo qualità	12.5% SDS-PAGE Stained with Coomassie Blue.
Tampone di conservazione	In modified RIPA Lysis Buffer.
Content And Storage	Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Suggerimenti di prodotto

Descrizione