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  6. TdB Labs AB 40 kDa DEAE-dextran for Transfection in Gene Therapy Studies

TdB Labs AB 40 kDa DEAE-dextran for Transfection in Gene Therapy Studies

Codice prodotto. 30216513
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30216513 10 g 10g
30216284 1 g 1g
30216425 100 g 100g
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Código de producto. 30216513 Proveedor TdB Labs AB N.º de proveedor DD40_10g

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Descripción

DEAE-dextran 40 kDa is a cationic polysaccharide widely used in transfection, viral enhancement assays, and nucleic acid delivery. Its balance of size and charge makes it a versatile tool in cell culture and biochemical interaction studies.

  • Product Overview: DEAE-dextran is a cationic, water-soluble polysaccharide modified with diethylaminoethyl groups. Known for its strong positive charge and high biocompatibility, DEAE-dextran is ideal for researchers and bioprocess engineers working with protein purification, nucleic acid complexation, or surface functionalization. Available across a wide molecular weight range to match different project needs.
  • Key Features:
    • Strong Cationic Properties: DEAE-modification provides consistent positive charge for robust binding to negatively charged biomolecules like proteins, DNA, and RNA.
    • Flexible Molecular Weight Range: Offered from 4 kDa up to 2000 kDa, giving options for fine-tuning binding strength, diffusion, and separation in your application.
    • High Solubility: Fully water-soluble powder allows rapid and easy preparation for chromatography, formulation, or complexation protocols.
    • Batch-to-Batch Consistency: Tight control over molecular weight and functionalization ensures reliable results across experiments and production runs.
    • Stable Storage: Dry powder format provides long shelf life without special storage conditions, helping to minimize lab downtime.
  • Why Choose DEAE-dextran:
    • For Protein Purification: DEAE-dextran is a proven choice for anion-exchange chromatography workflows, helping isolate or fractionate proteins under mild, biocompatible conditions.
    • For Complexation and Binding Studies: Ideal for creating complexes with nucleic acids or proteins to study binding kinetics, surface adsorption, or biomolecular interactions.
    • For Transfection Research: Historically used in mammalian cell DNA transfection experiments, DEAE-dextran still finds niche use where simple, non-viral gene delivery is studied.
    • For Surface Modification: Researchers functionalizing surfaces (e.g., biosensors, biomaterials) benefit from DEAE-dextran's ability to introduce stable, positive surface charges.
    • For Vaccine Adjuvant Studies: Studied as a component in veterinary vaccine formulations to enhance immune system targeting by improving antigen presentation.
  • Typical Applications:
    • Ion-exchange chromatography for proteins and nucleic acids
    • Formation of electrostatic complexes for analytical and purification purposes
    • Historical and research-based DNA transfection in mammalian cells
    • Surface coating and modification in bioengineering projects
    • Supportive studies in vaccine and adjuvant formulation development
  • Technical Details:
    • Molecular Weights: 4 kDa, 20 kDa, 40 kDa, 70 kDa, 150 kDa, 500 kDa, and 2000 kDa available.
    • Charge Density: Approximately 0.1 to 0.2 DEAE groups per glucose unit (depending on the grade).
    • Appearance: White to off-white dry powder, highly soluble in water.
  • Storage and Handling:
    • Store dry at room temperature in a sealed container to maintain stability.
    • Protect from moisture to preserve full activity over long-term storage.
  • Availability:
    • Supplied as research-grade powder, suitable for laboratory use and pilot-scale process development.
    • Available in small packs for R and D or bulk volumes for production environments.

Especificaciones

Tipo di cellula HEK293
Formato Vial
Promotore None (Promoterless)
Transfection Technique Chemical Transfection
Specie Human
Product Type Transfection Reagent
Quantità 10 g
Sterilità Non-sterile
Título del producto
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