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Abnova ES-E14TG2a (Mouse pluripotent embryonic stem cell) whole cell lysate (nondenatured)
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Quantità:
50 μg
Dimensione della confezione:
50µg
Beskrivelse
- Maintained in the undifferentiated state using specialized culturing protocols
- Monitored for undifferentiated cell morphology
- Analyzed using Flow Cytometry against specific cell marker
- Coommasie Blue Staining of total cell lysate in SDS-PAGE
Western Blotting, Immunoprecipitation
Tekniske data
Tekniske data
| Da utilizzare con (applicazione) | Immunoprecipitation, Western Blot |
| Tissue | Embryonic |
| Descrizione | Whole cell lysate (non-denatured) |
| Tampone di lisi | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Metodo di preparazione | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). |
| Analisi di controllo qualità | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quantità | 50 μg |
| Tampone di conservazione | In modified RIPA Lysis Buffer. |
| Specie ospite | Mouse |
| Concentrazione | 4 mg/mL |
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