TOKU-E™ Actinomycin D ReadyMade™ Solution

• CAS Number: 50-76-0
• Molecular Formula: C62H86N12O16
• Molecular Weight: 1255.42 g/mol
• Storage Conditions: -20°C
• Form: Liquid
• Source: Streptomyces sp.
• Spectrum: Actinomycin D has an inhibitory effect on Gram-positive, Gram-negative bacteria and some fungi. However, its toxicity precludes its use for infectious diseases
• Mechanism of Action: Actinomycin is a cyclic polypeptide-containing antibiotic that binds to DNA and inhibits RNA polymerase activity

As Actinomycin can bind DNA duplexes, it can also interfere with DNA replication, although other chemicals such as hydroxyurea are better suited for use in the laboratory as inhibitors of DNA synthesis. Actinomycin D and its fluorescent derivative,7-aminoactinomycin D (7-AAD), are used as stains in microscopy and flow cytometry applications. The affinity of these stains/compounds for GC-rich regions of DNA strands makes them excellent markers for DNA. 7-AAD binds to single stranded DNA; therefore, it is a useful tool in determining apoptosis and distinguishing between living and dead cells.