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Thermo Scientific™ ApaI (10 U/μL)

Codice prodotto. 10130690 Sfoglia Tutto Thermo Scientific Prodotti
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3000 U
5,000 units
Dimensione della confezione:
3000 unità
5000 unità
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10130690 3000 U 3000 unità
10550841 5,000 units 5000 unità
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Codice prodotto. 10130690 Fornitore Thermo Scientific™ N. del fornitore ER1411

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55.17 EUR valido fino al 2026-03-20
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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Descrizione

Cut at GGGCC^C sites with ApaI restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: PspOMI).

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

Incubation at 30°C results in a 2-fold increase in activity. ApaI is inhibited by salt concentrations above 50 mM. ApaI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.

TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 10 U/μL
Componenti 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
Temperatura incubatore 37°C
Quantità 3000 U
Product Type Apal

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
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