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BD Human IL-6 ELISA Kit II

Codice prodotto. 15856388
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Quantità:
2 Plates
Dimensione della confezione:
1 set
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Codice prodotto. Quantità unitSize
15856388 2 Plates 1 set
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Toxic, avoid contact with skin, eyes, or clothing
Codice prodotto. 15856388 Fornitore BD N. del fornitore 550799

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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
Toxic, avoid contact with skin, eyes, or clothing

Descrizione

Includes

2 plates of 96 breakable wells coated with anti-human IL-6 antibody; Detection antibody: Biotinylated anti-human IL-6; Enzyme Concentrate (250X): Horseradish peroxidase conjugate; Recombinant human IL-6, lyophilized, 4 vials; Standard Diluent; Wash Concentrate (20X); Substrate Reagent A; Substrate Reagent B; Stop Solution; Adhesive Plate Sealers

Requires

Microplate reader to read at 450nm, precision pipettes (50 and 100μL), adjustable 1mL, 5mL, 10mL, 25mL pipettes, deionized or distilled water, wash bottle or automated microplate washer, log-log graph paper or computer and software for ELISA data analysis, tubes to prepare standard dilutions, laboratory timer, absorbent paper

Quantitative determination of human IL-6 in serum, plasma, and cell culture supernatant

IL-6 specific monoclonal antibody binds to immobilized antibody coated on 96-wells plate. Wells are washed and streptavidinhorseradish peroxidase conjugate mixed with biotinylated anti-human IL-6 antibody is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-6 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450nm.

  • Solid phase sandwich ELISA
  • Standard Range: 4.7 - 300pg/mL
  • Sensitivity: 4pg/mL

TRUSTED_SUSTAINABILITY

Specifica

Antigene IL-6
Cross-reattività Human
Include 2 Plates of 96 breakable well coated with Anti-Human IL-6 Antibody, Biotinylated Anti-Human IL-6, HRP Conjugate (250X), 4 vials recombinant Human IL-6 (lyophilized), standard Diluent, wash concentrate (20X), Substrate reagent A, Substrate reagent B, stop
Quantità 2 Plates
Tipo di campione Serum, Plasma, Cell Culture
Content And Storage 2°C to 8°C
Da utilizzare con (applicazione) Quantitative determination of Human IL-6, ELISA
Product Type Human IL-6 ELISA kit II

Research use only

Titolo del prodotto
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