Thermo Scientific™ CaptureSelect™ LambdaXP GoPure™ Column

Features of this affinity matrix include:

• Platform approach for purification of immunoglobulins and their Fab fragments with a Lambda light chain
• Full coverage for all human Lambda subclasses
• One-step purification from crude material with high purity and yield
• No cross binding to the Fc region or kappa light chains
• Excellent pH stability at 50–80 mM NaOH for limited exposure
• Specific for human antibodies; works in the presence of serum (bovine/horse)
• Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

Matrix: agarose-based, epoxide-activated

Average particle size: 65 ± 10 μm

Ligand :CaptureSelect LambdaXP affinity ligand

Fab binding capacity: >20 mg IgG Lambda-Fab/mL resin, depending on residence time

IgG binding capacity: >35 mg IgG/mL resin, depending on residence time

Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl₂, pH 6

Column dimensions: 7 x 25 mm (1 mL column); 16 x 25 mm (5 mL column)

Max. recommended flow: 5 mL/min. (1 mL column); 10 mL/min. (5 mL column)

Max. operation pressure: 2 bar

Formulation buffer: 20% (v/v) ethanol