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Invitrogen™ Collibri™ 3´ mRNA Library Prep Kit for Illumina™ Systems

Designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) platforms.

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Specifica

Content And Storage • Collibri Library Prep Kit, store at -20°C
• Collibri Library Cleanup Kit, store at 4°C
Formato Kit
Sequencing Type Transcriptome Sequencing
Da utilizzare con (applicazione) Sequencing
Librerie cDNA Library
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Descrizione

Descrizione

  • Supports library preparation from polyadenylated RNA in ∼4.5 hours.
  • The protocol generates one fragment per transcript, resulting in accurate gene expression values.
  • Sequences obtained are close to the 3’ ends of the transcripts, so the kit is suitable for varying-quality
  • RNA samples, including formalin-fixed paraffin-embedded (FFPE) samples.
  • The majority of inserts are greater than 75 bp in size, corresponding to final library fragment sizes ≥200 bp.
  • Multiplexing of libraries can be carried out using up to 96 single-indexed primers.
  • For convenience, the Collibri 3’ mRNA Library Prep Kit provides color-coded components for visual tracking of library preparation progress.
  • Inert dyes in the reagents provide visual confirmation of additions and mixing, but do not interfere with enzymatic reactions and do not compromise library prep and sequencing results.

Specifica

Specifica

• Collibri Library Prep Kit, store at -20°C
• Collibri Library Cleanup Kit, store at 4°C
Transcriptome Sequencing
cDNA Library
mRNA Library Prep Kit
Library Generation
Kit
Sequencing
Collibri™
Approved for shipment on Wet or Dry Ice
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