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Thermo Scientific™ DMEM for SILAC

Codice prodotto. 15645729 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
500 mL
6 x 500 mL
Dimensione della confezione:
500mL
pezzi 6
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Codice prodotto. Quantità unitSize
15645729 500 mL 500mL
15786803 6 x 500 mL pezzi 6
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Product Code. 15645729 Supplier Thermo Scientific™ Supplier No. 88364

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Description

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ DMEM for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of DMEM for SILAC:

  • Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
  • High quality—medium is sterile, endotoxin-free, and cell culture-compatible

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.

This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Related products

  • NeuCode™ Lysine -080, 50 mg (A33613)
  • Fetal Bovine Serum, dialyzed, US origin (26400036)
  • RPMI 1640 Media for SILAC (88365)
TRUSTED_SUSTAINABILITY

Specifications

Da utilizzare con (apparecchiatura) Mass Spectrometer
Product Type Media
Content And Storage 1 x 500 mL, store at 2–8°C
Label or Dye Mass Isotope of Amino Acid
Shipping Condition Room Temperature
Starting Material Protein Samples
Tipo di prodotto finale Liquid Medium
Metodo di rilevamento Mass Spectrometry
Workflow Step Cell Culture
Quantità 500 mL
Forma Liquid
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For Research Use Only. Not for use in diagnostic procedures.

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