IBA Lifesciences pASG-IBA E. coli Expression Vector with Secretion

• The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.

• The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.

• The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.

• Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.

Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.