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Thermo Scientific™ Esonucleasi III (Exo III)
Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.
Marca: Thermo Scientific™ EN0191
33.65 EUR valido fino al 2024-12-31
Usa il codice promo "22915" per avere il tuo prezzo promozionale.
Dettagli aggiuntivi : Peso : 1.51950kg
Descrizione
Exonuclease III (ExoIII) exhibits four catalytic activities (see Reference1). The 3'=>5' exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA. ExoIII degrades dsDNA from blunt ends, 5'-overhangs or nicks, releases 5'-mononucleotides from the 3'-ends of DNA strands and produces stretches of single-stranded DNA. It is not active on 3'-overhang ends of DNA that are at least four-bases long and do not carry a 3'-terminal C-residue (see Reference 2) on single-stranded DNA, or on phosphorothioate-linked nucleotides.
ExoIII 3'-phosphatase activity removes the 3'-terminal phosphate, generating a 3'-OH group. ExoIII Rnase H activity exonucleolytically degrades the RNA strand in RNA-DNA hybrids. ExpOOO apurinic/apyrimidinic-endonuclease activity cleaves phosphodiester bonds at apurinic or apyrimidinic sites to produce 5'-termini that are base-free deoxyribose 5'-phosphate residues.
Highlights
- Active in restriction enzyme buffers
Specifica
| 200 U/μL | |
| Enzyme Buffer | |
| Exonuclease III |
| Exonuclease | |
| 4000 U |
For Research Use Only. Not for use in diagnostic procedures.