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Descrizione
- A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications
- The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules
- Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner
- Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately)
- The coupling or crosslinking procedure is typically done in two steps:
- First, a protein or thiol containing molecule is labeled with a DBCO group and excess crosslinker is removed
- Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety
- Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst
- Reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures
Specifica
Specifica
| Chemical Reactivity | Azide-Sulfhydryl |
| Cleavable | No |
| Descrizione | Maleimide-PEG4-DBCO |
| Peso molecolare | 647.74 |
| PEGylated | Yes |
| Lunghezza braccio spaziatore | 29.7 Å |
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Permeabilità celle | Not determined |
| Shipping Condition | Wet Ice |
| Linea di prodotti | EZ-Link No-Weigh |
| Vedi altri risultati |
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