missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Crosslinking, Labeling and Protein Modification
  5. Protein and Antibody Labeling Kits
  6. 10127773

Thermo Scientific™ EZ-Link™ NHS-PEG Solid-Phase Biotinylation Kit - 1mL Column

Codice prodotto. 10127773 Sfoglia Tutto Thermo Scientific Prodotti
Cambia vista
Click to view available options
Quantità:
8 Reactions
Dimensione della confezione:
1 set
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. Quantità unitSize
10127773 8 Reactions 1 set
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
Codice prodotto. 10127773 Fornitore Thermo Scientific™ N. del fornitore 21440

Effettua il per acquistare questo articolo Hai bisogno di un conto web ? Registrati oggi stesso!

Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

Descrizione

Biotinylate protein amines with greater control over the amine-labeling reaction and antibody cleanup process using this column-based method of antibody biotinylation.

Thermo Scientific™ EZ-Link NHS-PEG Solid Phase Biotinylation Kits provide an on-column approach to amine-targeted antibody biotinylation that provides greater control over the labeling reaction and clean-up process.

This innovative antibody-labeling system uses nickel-chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions. Once held in place on the resin, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and byproducts are then washed away before recovering the labeled and purified antibody from the resin using a mild imidazole solution. No gel filtration or dialysis is needed. Four kits are available for small (0.1-1mg) or large (1-10mg) antibody samples using either amine-directed (NHS ester) or sulfhydryl-directed (maleimide) labeling reagents.

Highlights:

  • Fast labeling and purification - the entire procedure takes only one hour (two hours for sulfhydryl labeling kits)

  • Easy removal of spent and excess labeling reagent - simply wash away the reaction byproducts - no need for dialysis or gel filtration

  • No dilution effects - solid-phase method allows initially dilute antibodies to be recovered in a smaller volume after labeling

  • Optimized protocols - specific protocols for antibody ensure appropriate level of labeling (2-5 biotins per antibody molecule), minimizing possibility of inactivation caused by overlabeling

  • Sufficient reagents for eight biotin-labeling experiments - Thermo Scientific No-Weigh Packaging ensures that the biotin reagent is fully active for eight separate experiments

Includes:

Immobilized Nickel Chelate Column 1mL, BupH Phosphate Buffered Saline 1 pack (makes 500mL), No-Weigh NHS-PEG4-Biotin Microtubes 8 x 2mg, 4M Imidazole Stock Solution 5mL

TRUSTED_SUSTAINABILITY

Specifica

Product Type NHS-PEG Solid-Phase Biotinylation Kit
Quantità 8 Reactions
Content And Storage Sufficient For: 8 labeling reactions, each with 1 to 10 mg of antibody
• Ni-IDA Agarose Column, 1 mL, 1 column
• PBS pack (makes 500mL), 1 pack
• NHS-PEG4-Biotin, 8 x 2 mg
• Imidazole Stock Solution (4M), 5 mL

Store at 4°C.
Labeling Scale 1 to 10 mg
Linea di prodotti EZ-Link
Label or Dye Biotin
Spacer Pegylated
Labeling Target IgG
Titolo del prodotto
Selezionare un problema

Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.