Thermo Scientific™ EZ-Link™ NHS-PEG₁₂-biotina

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging helps prevent the loss of reagent reactivity and contamination over time by minimizing the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

• Protein labeling: Biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Amine-reactive: Reacts with primary amines (-NH2), such as side-chain of lysines (K) or amino-termini of polypeptides
• PEGylated: Spacer arm contains hydrophilic, 12-unit, polyethylene glycol (PEG) group
• Enhances solubility: PEGylation imparts water solubility to biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible: Forms permanent amide bonds; spacer arm cannot be cleaved
• Very long reach: Spacer arm (total length added to target) is 56A⁰; this reduces steric hindrance when binding to avidin molecules