missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. Thermo Scientific™ BpiI (BbsI)

Thermo Scientific™ BpiI (BbsI)

Codice prodotto. p-4521470 Sfoglia Tutto Thermo Scientific Prodotti
Cambia vista
Click to view available options
Quantità:
1000 U
200 U
Dimensione della confezione:
1000 unità
200 unità
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. Quantità unitSize
10774491 200 U 200 unità
10609330 1000 U 1000 unità
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
Codice prodotto. 10774491 Fornitore Thermo Scientific™ N. del fornitore ER1011

Effettua il per acquistare questo articolo Hai bisogno di un conto web ? Registrati oggi stesso!

38.50 EUR valido fino al 2026-03-20
MIGLIOR PREZZO in promozione! Usa il codice promo "27968" per avere il tuo prezzo promozionale.
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

Descrizione

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G A A G A C (N)2▵...3'

3'...C T T C T G (N)6▵...5'

Conditions for 100% Activity:

  • 1X Buffer G
  • 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • BpiI is supplied in:
  • 10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BpiI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5 units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5ft.-overhangs. This feature is useful for direct PCR product cloning.

TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 10 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme BpiI (BbsI)
Compatible Buffer 10X Buffer G
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE Yes
Quantità 200 U
Product Type Restriction Enzyme
Research Category Scarless Cloning

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
Selezionare un problema

Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.