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Thermo Scientific™ Lambda DNA (dam-, dcm-)

Codice prodotto. 10252690 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
500 μg
Dimensione della confezione:
500µg
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Codice prodotto. Quantità unitSize
10252690 500 μg 500µg
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Codice prodotto. 10252690 Fornitore Thermo Scientific™ N. del fornitore SD0021

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53.00 EUR valido fino al 2026-03-20
MIGLIOR PREZZO in promozione! Usa il codice promo "27968" per avere il tuo prezzo promozionale.
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Descrizione

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5'-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination.

Highlights

Phage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is often used. The DNA sequence used to construct a phage alpha restriction map includes these mutations. The map shows enzymes that cut lambda DNA. Enzymes produced by Thermo Scientific are shown in orange (see Figure bellow).

Lambda DNA (dam-, dcm-) is isolated from a heat-inducible lysogenic E. coli GM2163 strain.

TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 0.3 μg/μL
Tipo di campione DNA
Tipo di prodotto finale DNA
Da utilizzare con (applicazione) CloningNucleic Acid Gel Electrophoresis
Include [other]
Product Type Lambda DNA
Quantità 500 μg
Specie Lambda phage

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
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