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Thermo Scientific™ DraI

Codice prodotto. 10679880 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
1,500 U
Dimensione della confezione:
1500 unità
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Codice prodotto. Quantità unitSize
10679880 1,500 U 1500 unità
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Codice prodotto. 10679880 Fornitore Thermo Scientific™ N. del fornitore ER0221

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55.67 EUR valido fino al 2026-03-20
MIGLIOR PREZZO in promozione! Usa il codice promo "27968" per avere il tuo prezzo promozionale.
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Descrizione

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

5'...T T T▵A A A...3'

3'...A A A▵T T T...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™:
  • 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • DraI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with DraI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: may overlap — blocked
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 10 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme Dra I
Compatible Buffer 10X Buffer Tango
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantità 1,500 U
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
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