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Descripción
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease activity of proofreading DNA polymerases, such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.
Features
• 3'-terminal PTO modifications protect from 3'→5' exonuclease degradation and ensure efficient priming of DNA synthesis
• Product delivered in a ready-to-use format
Applications
• Strand displacement amplification of genomic DNA, plasmids, and phage DNA
• DNA labeling by random priming
Especificaciones
Especificaciones
| Product Type | Primer |
| Content And Storage | 20X Exo-Resistant Random Primer (100 μL) Store at –20°C. |
| Shipping Condition | Dry Ice |
| Concentrazione | 500 μM |
| Primer | Random |
| Quantità | 100 μL |
| Da utilizzare con (applicazione) | DNA Labeling, Strand Displacement Amplification |
| Forma | Liquid |
For Research Use Only. Not for use in diagnostic procedures.
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