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Thermo Scientific™ HinfI, HC (50 U/μL)
Thermo Scientific™ Fermentas HinfI is available as a FastDigest enzyme which is used for rapid DNA digestion.
Marca: Thermo Scientific™ ER0803
Dettagli aggiuntivi : Peso : 0.01000kg
Descrizione
Thermo Scientific™ HinfI is available as a FastDigest enzyme which is used for rapid DNA digestion. More than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 1.3μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with HinfI.
5'...G▵A N T C...3'
3'...C T N A▵G...5' Conditions for 100% Activity:
- 1X Buffer R: 10mM Tris-HCl (pH8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer:
- HinfI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
Methylation Effects:
- Dam: never overlaps —no effect
- Dcm: never overlaps —no effect
- CpG: may overlap —cleavage impaired
- EcoKI: never overlaps —no effect
- EcoBI: may overlap —blocked
Compatible Ends:
- G▴AWTC — PfeI
Specifica
| 50 U/μL | |
| Hinf I | |
| Yes | |
| No | |
| Restriction Enzyme |
| CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive | |
| 10X Buffer R | |
| 37°C | |
| 10,000 U | |
| Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.