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Thermo Scientific™ HinfI

Codice prodotto. 10334580 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
2,000U
5 x 2,000 units
Dimensione della confezione:
2000 unità
pezzi 5
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Codice prodotto. Quantità unitSize
10334580 2,000U 2000 unità
11853833 5 x 2,000 units pezzi 5
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Codice prodotto. 10334580 Fornitore Thermo Scientific™ N. del fornitore ER0801

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Descrizione

The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.

Lambda DNA digested with HinfI, 1.4% agarose, 148 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G▵A N T C...3'

3'...C T N A▵G...5' Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HinfI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —blocked

Compatible Ends:

  • G▴AWTC — PfeI
TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 10 U/μL
Methylation Sensitivity CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme Hinf I
Compatible Buffer 10X Buffer R
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantità 2,000U
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
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