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  5. Thermo Scientific™ HpyF3I (DdeI) (10 U/μL)

Thermo Scientific™ HpyF3I (DdeI) (10 U/μL)

Codice prodotto. 10191030 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
2,500 units
500 units
Dimensione della confezione:
2500 unità
500 unità
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Codice prodotto. Quantità unitSize
10191030 500 units 500 unità
10889710 2,500 units 2500 unità
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Codice prodotto. 10191030 Fornitore Thermo Scientific™ N. del fornitore ER1881

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81.60 EUR valido fino al 2026-07-03
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Descrizione

The HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstDEI, DdeI).

Lambda DNA digested with HpyF3I (DdeI), 1.4% agarose, 104 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..C▵T N A G...3'

3'..G A N T▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HpyF3I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Compatible Ends:

  • BbvCI, Bpu10I, Bpu1102I, Eco81I
TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 10 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme HpyF3I (DdeI)
Compatible Buffer 10X Buffer Tango
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantità 500 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
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