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Thermo Scientific™ PstI
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Lambda DNA digested with PstI, 0.7% agarose, 28 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
5'..C T G C A▵G...3'
3'..G▵A C G T C...5'
Conditions for 100% Activity
- 1X Buffer O:50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer
- PstI is supplied in: 10mM Tris-HCl (pH7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol
Ligation and Recleavage
- After 50-fold overdigestion with PstI, more than 95% of the DNA fragments can be ligated and recut
Methylation Effects
- Dam: never overlaps —no effect
- Dcm: never overlaps —no effect
- CpG: never overlaps —no effect
- EcoKI: never overlaps —no effect
- EcoBI: never overlaps —no effect
Digestion of Agarose-embedded DNA
- Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
Compatible Ends
- Alw21I, ApaI, BseSI, Eco24I, Mph1103I, PstI, SacI, SdaI
Note:
Conditions of high pH, low salt, high glycerol, 8% DMSO can cause star activity (Malyguine, E., et al., Gene, 8, 163-177, 1980). Surrounding sequences: the presence of adjacent runs of G-C base pairs confers significant resistance to cleavage (Armstrong, K. and Bauer, W.R., NAR, 10, 993-1007, 1982). 100% dUTP incorporation at the recognition site reduces PstI cleavage to 25% (Glenn, T.C., et al., Biotechniques, 17, 1086-1090, 1994). PstI will not cut AGCTGCAG when methylated by AluI methyltransferase.
Spécification
Spécification
| Concentrazione | 10 U/μL |
| Methylation Sensitivity | Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
| Enzyme | Pst I |
| Compatible Buffer | 10X Buffer O |
| Sensitive to Heat Inactivation | No |
| Optimal Reaction Temperature | 37°C |
| Type IIS RE | No |
| Quantità | 5 x 3,000 units |
| Product Type | Restriction Enzyme |
| Research Category | Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.
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