Thermo Scientific™ pUC57 DNA

Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.

Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3'-ends that are resistant to E. coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact positions of the genetic elements are shown on the map (termination codons included). DNA replication initiates at position 890 (±1) and proceeds in the direction indicated. The bla gene nucleotides 2510-2442 (compl. strand) code for a signal peptide.