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Thermo Scientific™ PvuI

Codice prodotto. 10181170 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
1,500 units
300U
Dimensione della confezione:
1500 unità
300 unità
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Codice prodotto. Quantità unitSize
10181170 300U 300 unità
10141220 1,500 units 1500 unità
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
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Codice prodotto. 10181170 Fornitore Thermo Scientific™ N. del fornitore ER0621

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Descrizione

The PvuI restriction enzyme recognizes CGAT^CG sites and cuts best at 37°C in R buffer (Isoschizomers: BpvUI, MvrI, Ple19I).

Lambda DNA digested with PvuI, 0.7% agarose, 3 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..C G A T▵C G...3'

3'..G C▵T A G C...5'

Conditions for 100% Activity

  • 1X Buffer R:10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • PvuI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with PvuI, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recut

Methylation Effects

  • Dam: completely overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

  • Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, XmiI
TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 10 U/μL
Methylation Sensitivity CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme Pvu I
Compatible Buffer 10X Buffer R
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantità 300U
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
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