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Thermo Scientific™ RNasi T1
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
Marca: Thermo Scientific™ EN0542
Dettagli aggiuntivi : Peso : 0.00600kg
Descrizione
Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.
Rnase T1 does not require metal ions for activity.
Applications
- Removal of RNA from DNA preparations
- RNA sequencing
- Ribonuclease protection assays. Used in conjunction with RNase A
- Removal of RNA from recombinant protein preparations
- Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a "G-less cassette"
Specifica
| 1000 U/μL | |
| RNase | |
| 500,000 U | |
| RNase T1 |
For Research Use Only. Not for use in diagnostic procedures.