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Thermo Scientific™ SP6 RNA Polymerase (20 U/μL)

Codice prodotto. 10344450 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
2,000 units
Dimensione della confezione:
2000 unità
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Codice prodotto. 10344450

Marca: Thermo Scientific™ EP0131

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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

Descrizione

Thermo Scientific™ SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Thermo Scientific™ Bacteriophage SP6 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

TRUSTED_SUSTAINABILITY

Specifica

Polymerase SP6 RNA Polymerase
Quantità 2,000 units
Concentrazione 1X

For Research Use Only. Not for use in diagnostic procedures.

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