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Descrizione
Thermo Scientific Transcription Promoter Sequencing Primers are single-stranded oligonucleotides with 5'- and 3'-hydroxyl ends. The primers are complementary to the T3 RNA Polymearse promoter region and are supplied as 10 µM aqueous solutions.
Applications
• Sequencing of DNA fragments located downstream from the T3 RNA polymerase promoter sequence in common cloning vectors, such as pTZ19R (Cat. No. SD0141), pTZ57R, and pBluescript II
Promoter sequence 5'-d (ATTAACCCTCACTAAAG)-3'
Related products
T3 promoter Sequencing Primer, 24-mer (SO120)
T7 promoter Sequencing Primer, 20-mer (SO118)
SP6 promoter Sequencing Primer, 18-mer (SO116)
SP6 promoter Sequencing Primer, 24-mer (SO117)
Notes
• Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters
• Primers are not phosphorylated
Specifica
Specifica
| Promotore | SP6, T3, T7 |
| Product Type | Sequencing Primer |
| Content And Storage | T3 promoter Sequencing Primer, 17-mer, 10 μM (60 μL) Store at –20°C. |
| Shipping Condition | Dry Ice |
| Concentrazione | 10 μM |
| Primer | T3 |
| Quantità | 10 μM, 60 μL |
| Vettore | pTZ19R, pTZ57R, pBluescript II |
| Da utilizzare con (applicazione) | Sequencing |
| Forma | Liquid |
For Research Use Only. Not for use in diagnostic procedures.
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