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Cytiva Amersham™ Megaprime™ DNA Labeling System
For labeling DNA probes to specific activities >1 x 109dpm/μg in 10 minutes
Marca: Cytiva RPN1604
Dettagli aggiuntivi : Peso : 0.12100kg
Includes: Primer solution; Labeling buffer; Reaction buffer; Enzyme; Standard DNA solution; Carrier DNA solution
Descrizione
Megaprime™ DNA Labeling Systems are used for rapid, random-prime labeling from as little as 25ng substrate DNA.
- Features cloned Klenow fragment and random nonamers in the random-prime labeling technique
- No need to remove unincorporated nucleotides before hybridization
- Protocols for labeling directly from low melting point agarose
Specifica
| -15°C to -30°C | |
| Primer solution 150μL; Labeling Buffer dATP, dGTP and dTTP in Tris HCl pH7.5, 2-mercaptoethanol and MgCl2 Nucleotide solutions: dATP, cCTP, dGTP, dTTP in Tris HCl pH 8, 0.5mM EDTA 120μL, Reaction Buffer A 10 x concentra | |
| Megaprime DNA Labeling System, dNTP (for use with any radioactively labeled nucleotide) |
| Membrane hybridization | |
| 30 |