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Cytiva TempliPhi™ 2000 Reaction Kit
Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications
Marca: Cytiva 28-9642-86
Dettagli aggiuntivi : Peso : 1.81440kg
Includes: TempliPhi Premix, Denature buffer, Enzyme mix, and Positive control pUC19 DNA (2ng/μL)
Descrizione
- Simple protocol reduces time, labor and consumables needed for template preparation, and workflow enables easy automation
- Generate microgram quantities of template DNA from picogram amounts of starting material
- Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks or purified circular (plasmid or M13) DNA
- Use amplified DNA directly for cycle sequencing without purification
- Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h
Specifica
| Store Denature buffer and control DNA at -70°C or -15°C to -30°C. Store TempliPhi premix at -70°C.Do not store TempliPhi premix at -15°C to -30°C. Thaw the premix on ice and maintain at 0–4°C during handling. Do not allow premix to warm above 4°C prior | |
| High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation. | |
| 2000 | |
| 16-18 h |
| illustra TempliPhi 2000 Reaction Kit | |
| TempliPhi™ premix, denature Buffer, Enzyme mix and positive control pUC19 DNA | |
| illustra TempliPhi 2000 Reaction Kit |