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Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 680, Invitrogen™

Goat Polyclonal Secondary Antibody

Marca:  Invitrogen A32734

349.80 EUR valido fino al 2024-03-29
Usa il codice promo "21653" per avere il tuo prezzo promozionale.



Dettagli aggiuntivi : Peso : 0.01000kg

Codice prodotto. 15657898

  • 373.00€ / 1mg
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Descrizione

Descrizione

To minimize cross-reactivity, the goat anti-rabbit IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Rabbit IgG (H+L) Highly Cross-Adsorbed Polyclonal antibody specifically detects Rabbit IgG (H+L) Highly Cross-Adsorbed in Rabbit samples. It is validated for Flow Cytometry, Immunocytochemistry, Western Blot.
Specifica

Specifica

Rabbit IgG (H+L) Highly Cross-Adsorbed
Polyclonal
Alexa Fluor Plus 680
Goat
Affinity chromatography
RUO
Rabbit
Liquid
Flow Cytometry, Immunocytochemistry, Western Blot
2 mg/mL
proprietary buffer with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone; pH 6.5
Gamma Immunoglobins Heavy and Light chains
1 mg
Secondary
4° C, store in dark
IgG
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