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Thermo Scientific™ pTK-Green Renilla Luc Vector for Luciferase Assays
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Drive Green Renilla luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pTK-Green Renilla Luc vector:
- Weak (TK) constitutive promoter for co-transfection and normalization
- Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
- Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
- Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
- High-copy pUC bacterial DNA replication origin
Related Products
pMCS-Green Renilla Luc Vector for Luciferase Assays (Cat. No. 16152)
pCMV-Green Renilla Luc Vector for Luciferase Assays (Cat. No. 16153)
Specifications
Specifications
| Descrizione | pTK-Green Renilla Luc Vector |
| Tipo | Luc Vector |
| Cloning Method | Restriction Enzyme/MCS |
| Tipo di erogazione | Transfection |
| Quantità | 10 μg |
| Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C. |
| Gene reporter | Green Renilla Luciferase |
| Agente di selezione (eucariotico) | Puromycin |
| Linea di prodotti | Pierce |
| Da utilizzare con (applicazione) | Reporter Assays |
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For Research Use Only. Not for use in diagnostic procedures.
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