MP Biomedicals™ Ham's F-12 (modified) with L-Glutamine, without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 mediums are formulated for use with or without serum, depending on the type of cells being cultured.

Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells, and is also the medium of choice for toxicity assays using CHO cells. MP Biomedical™ also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2 to 7.4.

F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modified (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells and primary human liver cells. MgCl₂ has been substituted by MgSO₄ containing an equivalent amount of Mg ion.

Usage Statement: Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.