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HCT 116 (human colorectal carcinoma) Whole cell lysate, Non-denatured; Abnova

Product Code. 16019635
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Quantità:
200 μg
Unit Size:
200µg
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16019635 200 μg 200µg
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Product Code. 16019635 Supplier Abnova Supplier No. L052V2.200ug

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Description

Western Blotting, Immunoprecipitation

Specifications

Da utilizzare con (applicazione) Immunoprecipitation, Western Blot
Tissue Colon, Colorectal Carcinoma
Descrizione Whole cell lysate (non-denatured)
Tampone di lisi Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Metodo di preparazione Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Analisi di controllo qualità 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantità 200 μg
Tampone di conservazione In modified RIPA Lysis Buffer.
Specie ospite Human
Concentrazione 5 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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