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HepG2/C3A (human hepatoblastoma) Nuclear extract lysate, Non-denatured; Abnova

Codice prodotto. 16049555
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Quantità:
50 μg
Dimensione della confezione:
50µg
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Codice prodotto. Quantità unitSize
16049555 50 μg 50µg
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Codice prodotto. 16049555 Fornitore Abnova N. del fornitore L019V4.50ug

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Descrizione

  • Tissue: Liver
  • Host: Human
  • Lysis buffer: Buffer A (10mM HEPES pH 7.9, 1.5mM MgCl2, 10mM KCl, 0.5 mM DTT), Buffer C (20mM HEPES pH 7.9, 25%(v/v) Glycerol , 0.42M NaCl , 1.5mM MgCl2, 0.2mM EDTA, 0.5mM DTT & 0.5mM PMSF), Buffer D (20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2mM EDTA, 0.5mM DTT & 0.5mM PMSF)
  • Storage buffer: In buffer D

Applications:

Western Blotting, Immunoprecipitation

Specifica

Da utilizzare con (applicazione) Immunoprecipitation, Western Blot
Tissue Liver
Descrizione Nuclear extract cell lysate (non-denatured)
Tampone di lisi Buffer A: 10mM HEPES pH 7.9, 1.5mM MgCl2, 10mM KCl, 0.5 mM DTT. Buffer C: 20mM HEPES pH 7.9, 25%(v/v) Glycerol , 0.42M NaCl , 1.5mM MgCl2, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF. Buffer D : 20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF.
Metodo di preparazione Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C, and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged, divided into aliquots, and stored at -80°C. The protein concentration was determined by the method of Bradford (Bio-Rad protein assay, microplate standard assay). The lysate was adjusted to 2 mg/ml.
Analisi di controllo qualità 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantità 50 μg
Tampone di conservazione In Buffer D.
Specie ospite Human
Concentrazione 2.5 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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