missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. Target-Specific Western Blot Kits
  5. 10573364

Thermo Scientific™ SuperSignal™ West HisProbe Kit

Codice prodotto. 10573364 Sfoglia Tutto Thermo Scientific Prodotti
Cambia vista
Click to view available options
Quantità:
685 mL
Dimensione della confezione:
1 set
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. Quantità unitSize
10573364 685 mL 1 set
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
Codice prodotto. 10573364 Fornitore Thermo Scientific™ N. del fornitore 15168

Effettua il per acquistare questo articolo Hai bisogno di un conto web ? Registrati oggi stesso!

Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

Descrizione

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

Thermo Scientific™ HisProbe-HRP is an nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP). This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins.

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).

Highlights:

  • Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol

  • Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies

  • High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags

  • Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

TRUSTED_SUSTAINABILITY

Specifica

Descrizione Pierce SuperSignal West HisProbe Kit
Product Type HisProbe Detection Kit
Metodo di rilevamento Chemiluminescence
Da utilizzare con (applicazione) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line HisProbe, SuperSignal
Quantità 685 mL
Coniugato HRP
Substrate Horseradish Peroxidase
Reattività All species
Sufficiente per 62 mini blots or 5000 sq cm membrane
Content And Storage Sufficient For: 62 mini blots or 5000 cm2 membrane
• HisProbe-HRP Conjugate, 2 mg
• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL
• SuperSignal West Pico Stable Peroxide Solution, 250 mL
• Blocker BSA in TBS (10X), 125 mL
• TBS Packs (each makes 500 mL), 10 packs
• Surfact-Amps 20 Detergent Solution, 6 x 10 mL

Store HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4°C. Store remaining components at 4°C.
Vedi altri risultati Mostra meno risultati

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
Selezionare un problema

Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.