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  6. Thermo Scientific™ Agarosio HisPur™ Ni-NTA Superflow

Thermo Scientific™ Agarosio HisPur™ Ni-NTA Superflow

Codice prodotto. 13414199 Sfoglia Tutto Thermo Scientific Prodotti
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Codice prodotto. 13414199 Fornitore Thermo Scientific™ N. del fornitore 25217

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Beschreibung

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Additional Information and Online Ordering

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

TRUSTED_SUSTAINABILITY

Spezifikation

Capacità (sistema metrico) 20 mg His-Tagged GFP Binding per mL Resin
Content And Storage Store at 4°C
Forma Liquid Suspension
Product Line HisPur
Quantità 1 L
Bersaglio His-Tagged Protein
Tipo Agarose
Fase stazionaria Divalent Nickel, Metal Chelate
Formato Bottle
Tipo di colonna Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.

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