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HL-60 (human promyelocytic leukemia) Whole cell lysate, Non-denatured; Abnova
Non-denatured whole cell lysate of HL-60 (human promyelocytic leukemia)
Marca: Abnova L014V2.200ug
Dettagli aggiuntivi : Peso : 0.02000kg
Descrizione
- Tissue: Peripheral Blood
- Host: Human
- Lysis Buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
- Storage buffer: In modified RIPA lysis buffer
Western Blotting
Specifica
| Western Blot | |
| Whole cell lysate (non-denatured) | |
| Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). | |
| 200 μg | |
| Human |
| Blood, peripheral | |
| Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In modified RIPA Lysis Buffer. | |
| Store at -80°C. Aliquot to avoid repeated freezing and thawing. |