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  5. Invitrogen™ Human IL-6 ELISA Kit

Invitrogen™ Human IL-6 ELISA Kit

Codice prodotto. 10107683
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Quantità:
2 Plate Kit
5 Plate Kit
Dimensione della confezione:
1 set
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Codice prodotto. 10107683

Marca: Invitrogen™ EH2IL62

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Descrizione

ELISA

The Human Interleukin-6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

These human IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human interleukin-6 (IL-6)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 10.2 to 400pg/mL

  • Sensitivity: < 1pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFα, IFNγ, IFNα, GM-CSF, or mouse IL-6

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

TRUSTED_SUSTAINABILITY

Specifica

Intervallo saggio 10.2 to 400pg/mL
Assay Sensitivity <1pg/mL
Descrizione Human IL-6 ELISA Kit
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Tipo di campione Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine
Specificità No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6
Sufficiente per 2 x 96 microplate wells
Contenuto kit Biotinylated Antibody Reagent, 2 x 8mL, Anti-Human IL-6 Precoated 96-well Strip Plate, 2 ea, Adhesive Plate Covers, 12 ea, 30X Wash Buffer, 2 x 50mL, HRP Dilution Buffer, 2 x 14mL, Recombinant Human IL-6 Standard, 4 vials, Standard Diluent, 2 x 25mL, Stop Solution, 2 x 13mL, TMB Substrate Solution, 2 x 13mL
Misurazione Absorbance at 450nm minus absorbance at 550nm
Precisione Intra-assay CV <10%; inter-assay CV <10%
Quantità 2 Plate Kit
Volume del campione 50 μL
Bersaglio Natural and recombinant human interleukin-6 (IL-6)
Tempo totale saggio 3 hr.
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