Learn More
Descrizione
Thermo Scientific IMDM Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).
Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy” 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.
This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.
Features of IMDM Media for SILAC:
- Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
- High quality—media are sterile, endotoxin-free and cell culture-compatible
Specifica
Specifica
| Da utilizzare con (apparecchiatura) | Mass Spectrometer |
| Product Type | Media |
| Content And Storage | 1 x 500 mL, store at 2–8°C |
| Label or Dye | Mass Isotope of Amino Acid |
| Shipping Condition | Room Temperature |
| Starting Material | Protein Samples |
| Tipo di prodotto finale | Liquid Medium |
| Metodo di rilevamento | Mass Spectrometry |
| Workflow Step | Cell Culture |
| Quantità | 500 mL |
| Vedi altri risultati |
For Research Use Only. Not for use in diagnostic procedures.
Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.