Thermo Scientific™ Imject™ EDC BSA Spin Kit

Imject BSA is purified and lyophilized in buffers that optimize its stability and solubility for hapten conjugation. The large, single polypeptide protein contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers.

Preparations in phosphate buffer are ready for hapten-carrier conjugation via amine-reactive NHS-ester or glutaraldehyde crosslinking. Preparations in MES buffer are optimized for peptide-carrier conjugation via the carboxyl-reactive carbodiimide crosslinker called EDC (or EDAC). The Imject EDC Spin Kit includes ready-to-use units of BSA carrier protein, optimized reaction buffers and trouble-free spin desalting columns for immunogen preparation using the EDC method.

Highlights:

• Convenient packages – choose lyophilized BSA or complete EDC conjugation kit

• High-yield conjugation – each molecule of 59 lysine residues, 30 to 35 of which have primary amines that are capable of reacting with a conjugation reagent

• Validated quality – purified BSA (Fraction V) is available lyophilized and stabilized in PBS or MES buffer, ready for use in glutaraldehyde, NHS-ester or EDC crosslinking methods

• Compatible solubility – numerous carboxyl groups account for the net negative charge (pI 5.1) of BSA and its high solubility, allowing its use in a broad range of conjugation conditions

• Immunogenic – at 67kDa, BSA is sufficiently large to function as the primary immunogen or it can be used as an irrelevant protein carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten

Includes:

Kit contains lyophilized BSA (in MES buffer), EDC crosslinker, buffers and desalting to easily prepare ready-to-use immunogens for injection