Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, high ROX

Descrizione

Thermo Scientific™ Luminaris Color Probe qPCR Master Mixes are ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms.

• Specificity no primer dimers

• Sensitivitity detects low copy number targets

• Wide linear range accurate quantification across 9 orders of magnitude

• Reproducibility and convenience ready-to-use 2X master mix

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Excellent qPCR performance
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Includes:

• Luminaris Color Probe qPCR Master Mixes include Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye
• 40X Yellow Sample Buffer with a yellow dye
• Nuclease-free water

Recommended for:

Gene expression analysis; siRNA validation; Genotyping; Pathogen detection