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Beskrivelse
- Can be used for the purification of strep-tagged fusion proteins in batch in single or high-throughput format.
- Beads show low non-specific protein binding due to their agarose surface and high specificity of Strep-Tactin™ XT towards Strep-tag. This results in outstanding target protein purity and makes the MagStrep beads a perfect tool for the isolation of proteins or the identification of complexes via pull-downs, immunoprecipitation (IP), or co-immunoprecipitation (Co-IP).
- MagStrep Strep-Tactin™ XT beads are suitable for fast small-scale purifications in reaction tubes as well as 96-well plates.
- The beads can be easily and swiftly separated with a magnetic separator. This eliminates the need for centrifugation and reduces the risk of disturbing the pellet or loss of beads within the wash step to zero.
- The elution of target proteins or complexes can be triggered under mild conditions with the biotin-containing Buffer BXT or under denaturing conditions by boiling in SDS gel loading buffer.
Tekniske data
Tekniske data
| Capacità (sistema metrico) | 10 mL |
| Formulazione | 5% (v/v) Suspension in PBS with 0.1% (w/v) Sodium Azide |
| Support Type | 6% Agarose, Crosslinked |
| Dimensione particelle | 20 to 40 μm |
| Forma | Resin Slurry |
| Bersaglio | Twin-Strep-tag or Strep-tag II |
| Tipo | Magnetic Agarose Bead |
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