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  5. IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion

IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
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Codice prodotto. 15643868 Sfoglia Tutto IBA Lifesciences Prodotti
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Vettore:
pCSG-IBA103
pCSG-IBA105
pCSG-IBA123
pCSG-IBA143
pCSG-IBA145
pCSG-IBA162
pCSG-IBA164
pCSG-IBA167
pCSG-IBA168
pCSG-IBA23
pCSG-IBA3
pCSG-IBA43
pCSG-IBA45
pCSG-IBA5
pCSG-IBA62
pCSG-IBA64
pCSG-IBAwt1
Dimensione della confezione:
5µg
17 product options available for selection
Product selection table with 17 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. Vettore unitSize
15643868 pCSG-IBA103 5µg
15613858 pCSG-IBA3 5µg
15613868 pCSG-IBA64 5µg
15613878 pCSG-IBA145 5µg
15623858 pCSG-IBA5 5µg
15623878 pCSG-IBA162 5µg
15633858 pCSG-IBA23 5µg
15633878 pCSG-IBA164 5µg
15643878 pCSG-IBA167 5µg
15653878 pCSG-IBA168 5µg
15663868 pCSG-IBA105 5µg
15673858 pCSG-IBA43 5µg
15673868 pCSG-IBA123 5µg
15683858 pCSG-IBA45 5µg
15693848 pCSG-IBAwt1 5µg
15693858 pCSG-IBA62 5µg
15693868 pCSG-IBA143 5µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 17 options available.
17 options
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Codice prodotto. 15643868 Fornitore IBA Lifesciences N. del fornitore 55103001

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Descrizione

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

pCSG-IBA mammalian expression vectors

  • Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
  • Stable cell lines can be selected by the neomycin resistance gene (NeoR).
  • In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Specifica

Sistema costitutivo o inducibile Constitutive
Gene marcatore Ampicillin
Promotore CMV
Product Type Vector
Agente di selezione (eucariotico) Geneticin™ (G-418)
Stabilità 12 months after shipping
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Specie Mammalian
Shipping Condition Room temperature
Protein Tag Twin-Strep-tag
Cloning Method Direct Cloning
Concentrazione 250 ng/μL
Primer Forward
Quantità 5 μg
Vettore pCSG-IBA103
Da utilizzare con (applicazione) Episomal expression (oriP, EBNA-1)
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