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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
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280.00€
253.10€ / 5µg
Risparmia 26.90€
9% Sconto
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Descrizione
pCSG-IBA mammalian expression vectors
- Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
- Stable cell lines can be selected by the neomycin resistance gene (NeoR).
- In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.
Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.
Spécification
Spécification
| Sistema costitutivo o inducibile | Constitutive |
| Descrizione | pCSG-IBAwt1 is a large expression vector with universal features for transient expression as well as for generation of stable mammalian cell lines. |
| Gene marcatore | Ampicillin |
| Promotore | CMV |
| Product Type | Vector |
| Agente di selezione (eucariotico) | Geneticin™ (G-418) |
| Content And Storage | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Specie | Mammalian |
| Protein Tag | Untagged |
| Cloning Method | Direct Cloning |
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