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Mouse heart tissue lysate, Denatured; Abnova
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Quantità:
200 μg
Dimensione della confezione:
200µg
Descrizione
Western Blotting
Specifica
Specifica
| Da utilizzare con (applicazione) | Western Blot |
| Tissue | Heart |
| Descrizione | Whole tissue lysate (denatured) |
| Tampone di lisi | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. ;Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Metodo di preparazione | Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. |
| Analisi di controllo qualità | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quantità | 200 μg |
| Tampone di conservazione | In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). |
| Specie ospite | Mouse |
| Content And Storage | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
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