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Mouse spleen tissue lysate, Denatured; Abnova

Codice prodotto. 16089585
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Quantità:
200 μg
Dimensione della confezione:
200µg
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Codice prodotto. Quantità unitSize
16089585 200 μg 200µg
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Codice prodotto. 16089585 Fornitore Abnova N. del fornitore L034W1.200ug
 Articolo soggetto a restrizioni
Spedizione stimata: 09-09-2026
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Descrizione

  • Tissue: Spleen
  • Host: Mouse
  • Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In ready-to-use 1X sample buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Western Blotting

Specifica

Da utilizzare con (applicazione) Western Blot
Tissue Spleen
Descrizione Whole tissue lysate (denatured)
Tampone di lisi Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. ;Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Metodo di preparazione Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Analisi di controllo qualità 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantità 200 μg
Tampone di conservazione In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Specie ospite Mouse
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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