Thermo Scientific™ Mycoplasma / Ureaplasma Agar

Mycoplasma and Ureaplasma species are parasites on the surface of human and animal epithelium cells. Because some metabolic pathways are missing they are completely dependent on their hosts which deliver them the essential growth factors. So besides the rich peptone base, the medium includes the necessary nutrients (Vitox, cystein, yeast extract, urea and horse serum) which are in vivo offered by the host. U. urealyticum metabolizes urea which results in a pH shift to alkaline conditions. Therefore, manganese sulphate is oxidised into manganese oxide which is incorporated by U. urealyticum giving the dark brown color to the colonies. The pH shift leads to the color shift of the pH indicator phenol red. As a consequence the medium around the Ureaplasma colonies turns from yellow into red.

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