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Thermo Scientific™ Reagente di estrazione di proteine neuronali N-PER™

Artikelnummer. 12201812 Alle ansehen Thermo Scientific Produkte
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Quantità:
100 mL
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100mL
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Artikelnummer. 12201812

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Collect proteins from all cellular compartments of neuronal tissue and primary cultured neurons with this proprietary, neuronal-specific cell lysis reagent.

Thermo Scientific™ N-PER Neuronal Protein Extraction Reagent is a proprietary cell lysis reagent optimized for efficient extraction of proteins from all cellular compartments of neuronal tissue and primary cultured neurons.

Neuron cell lysates prepared with the N-PER Reagent may be used for enzymatic activity assays (e.g., Phosphatase, Kinase, ATPase Assays), immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purification. The N-PER Lysis Reagent enables protein extraction to be completed in less than 30 minutes and requires mechanical disruption of tissue (e.g., Dounce Homogenization, Polytron). Typical neuronal protein yields are 70-90μg of protein per mg of brain tissue or 300μg of total protein from 106 primary neuronal cells.

Highlights:

  • Optimized – efficient extraction of total neuronal protein, including membrane proteins, from tissue or primary cultured cells

  • Gentle – preserves protein function without compromising yield

  • Versatile – can be supplemented with protease inhibitors, reducing or chelating agents or required cofactors

  • Compatible – extracts are suitable for use with total protein, enzymatic and immunological assays and protein purification methods

Recommended for:

Isolate functional proteins from neuronal tissues or primary neuronal cells; Study enzymatic activity assays specific to neuronal tissue; Generate lysates from neuronal tissues for biomarker analysis

TRUSTED_SUSTAINABILITY

Spezifikation

Quantità 100 mL
Formato Liquid
Product Type Neuronal Protein Extraction Reagent
Volume (sistema metrico) 100 mL
Content And Storage Upon receipt store product at 4°C.
Product Line NE-PER

For Research Use Only. Not for use in diagnostic procedures.

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