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Invitrogen™ IL-12 p40 Rat ELISA Kit

A lymphokine

dietro preventivo

Specifica

Numero di accesso Q9R103
Intervallo saggio 15.6-1000 pg/mL
Assay Sensitivity <3 pg/mL
Coniugato Biotin
Product Type ELISA Kit
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Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Descrizione

Descrizione

The Rat Interleukin 12+p40 (Rt IL-12+p40) ELISA quantitates Rt IL-12+p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IL-12+p40. Principle of the method The Rat IL-12+p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The IL-12 Rat ELISA Kit quantifies natural and recombinant rat IL-12 heterodimer and the free p40 unit in serum, EDTA plasma, buffered solution, or cell culture medium.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Wet ice

Specifica

Specifica

Q9R103
<3 pg/mL
ELISA Kit
Rat
Colorimetric Microplate Reader
Il12a, Il12b
4.2%
HRP
RUO
2°C to 8°C
1 hr. 20 min.
15.6-1000 pg/mL
Biotin
Plasma, Serum, Supernatant
Colorimetric
64546, 84405
5.1%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
<3pg/mL
Plasma, 10 μL; Serum, 10 μL; Supernatant, 100 μL
Rat
4 hr.
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For Research Use Only. Not for use in diagnostic procedures.