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Descrizione
The pCL-10A1 packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a sell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of at the rate of about 1 infectious particle/cell/day Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. pCL-10A1 expresses 10A1 envelope protein that was isolated from a mouse infected with amphotropic MuLV (2,3). Retroviruses obtained by cotransfection with pCL-10A1 vector will infect most mammalian cells including hamster cells.
- Species: Human
- Specificity: Ectotropic (usually) (MoMuLV) Mouse and rat cells only (not human), Amphotropic (from 4070A MuLV) Most mammalian cells (but not hamster) 10A1 (MuLV) Most mammalian cells (including hamster)
- For large scale production, this plasmid may be amplified on LB plates containing 50μg/mL ampicillin
- Buffer: 10μg in 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Recommended Storage
Store at -20°C. Avoid freeze-thaw cycles.
Specifica
Specifica
| Tampone di conservazione | 10 μg in 1 x TE (10 mM Tris, pH 7.5, 1 mM EDTA) |
| Content And Storage | Store at -20 C. Avoid freeze-thaw cycles. |
| Specie | Mouse, Mammal |
| Concentrazione | 0.3 mg/mL |
| Quantità | 10 μg |
| Vettore | pCL-10A1 Retrovirus Packaging Vector |
| Da utilizzare con (applicazione) | AMPLIFICATION: The Plasmid DNA Contains an Ampicillin Resistant Gene. For Large Scale Production, This Plasmid May be Amplified on LB Plates Containing 50 ug/ml Ampicillin. The Liquid Cultures May be Grown (at 100 ug Ampicillin/ml. Use in Retroviral Production Reported in Multiple Pieces of Scientific Literature.) |
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